Exposure of HEp-2 Cells to Stress Conditions Influences Antinuclear Antibody Reactivity
نویسندگان
چکیده
منابع مشابه
Automated Indirect Immunofluorescence Evaluation of Antinuclear Autoantibodies on HEp-2 Cells
Indirect immunofluorescence (IIF) on human epithelial (HEp-2) cells is considered as the gold standard screening method for the detection of antinuclear autoantibodies (ANA). However, in terms of automation and standardization, it has not been able to keep pace with most other analytical techniques used in diagnostic laboratories. Although there are already some automation solutions for IIF inc...
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The American College of Rheumatology position statement on ANA testing stipulates the use of IIF as the gold standard method for ANA screening(1). Although IIF is an excellent screening test in expert hands, the technical difficulties of processing and reading IIF slides--such as the labor intensive slide processing, manual reading, the need for experienced, trained technologists and the use of...
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Antibody prepared against HEp-2 cells inhibited the replication of echovirus I. The number of infective centres was reduced and the cells which were infected produced less virus per cell than control cells. The attachment of virus to cells was not affected. The inhibition was reduced by increasing the multiplicity of infection. Application of antibody 5 min. after virus adsorption at 4 ° reduce...
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During the 12th International Workshop on Autoantibodies and Autoimmunity held in Sao Paulo, Brazil, on August 28, 2014, a full day session was devoted to establishing a consensus on the nomenclature of staining patterns observed in the antinuclear antibody (ANA) indirect immunofluorescence test on HEp-2 cells. The current report summarizes the collective agreements with input from the host Bra...
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Stress has significant consequences throughout the lifetime. However, when it occurs early in life, the implications may be particularly profound and long lasting. Evidence suggests that high levels of maternal stress during pregnancy are associated with alterations in the normal activity of the maternal hypothlalamic-pituitary-adrenocortical (HPA) and placental axis. Increased activity of this...
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ژورنال
عنوان ژورنال: Clinical and Vaccine Immunology
سال: 2002
ISSN: 1556-6811,1556-679X
DOI: 10.1128/cdli.9.2.287-294.2002